Protein Production and Purification

We produce high quality proteins, custom designed to meet the needs of our clients, for use in applications including X-ray crystallography, biophysical characterization, high-throughput screening, assay development, and pilot studies.

CONSTRUCT DESIGN AND EXPRESSION

We offer construct design and synthesis with codon optimization using bacterial, baculovirus, yeast, and mammalian expression systems.

PROTEIN PURIFICATION

We custom design purification methods to reach rigorous standards of protein purity, employing affinity-based techniques, chromatography, and refolding. We are experienced with challenging proteins, the assembly of higher order complexes, and the purification of proteins from natural sources.

CHARACTERIZATION

Our analysis of protein purity includes SDS-PAGE, Western blot analysis, mass spectrometry, size-exclusion chromatography, and thermal stability profiling. Our rigorous standards include purity at the level of sequence, quaternary structure, and post-translational modification.

Using thermal shift assay (TSA) analysis (also known as differential scanning fluorimetry, or DSF), we offer mapping of protein stability as a function of buffer, pH, salt concentration, and additives.

XTAL PROTEIN LISt (PDF)

We have approximately 150 high purity proteins available immediately for X-ray crystallography, biophysical characterization, screening, or assay development applications.